甾烷类化合物分子印迹聚合物功能单体的筛选及MIPs制备

Screening of functional monomers and preparation of molecularly imprinted polymers (MIPs) in molecularly imprinted polymers of steranes

马荣 原陇苗 刘艳红 王之语 吴应琴

MA Rong YUAN Longmiao LIU Yanhong WANG Zhiyu WU Yingqin

中国科学院西北生态环境资源研究院/甘肃省油气资源研究重点实验室, 兰州 730000 中国科学院大学, 北京 100049 海洋油气勘探国家工程中心, 北京 100028

Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu 730000, China University of Chinese Academy of Sciences, Beijing 100049, China National Engineering Research Center for Offshore Oil and Gas Exploration, Beijing 100028, China

为了制备对甾烷类化合物具有特异性选择能力的分子印迹聚合物(MIPs)，利用紫外光谱法对预聚合体系进行筛选，确定功能单体的种类、配比及其作用方式。将3种模板分子(胆固醇、β-谷固醇、去氧胆酸)分别与4种功能单体，即丙烯酸(AA)、甲基丙烯酸(MAA)、甲基丙烯酸甲酯(MMA)、丙烯酰胺(AM))的相互作用强度进行对比。研究结果发现，功能单体AA均可与3种模板分子发生较强的相互作用并形成稳定的预聚合体系，从而优选出AA为MIPs的功能单体。另外，通过不同比例AA紫外光谱吸光度变化和差示紫外光谱分析，显示3种模板分子与功能单体AA的最佳浓度比均为1∶4，且形成的稳定配合物构型分别为胆固醇-1AA、β-谷固醇-1AA及去氧胆酸-3AA。同时，以EDGMA为交联剂、AIBN为引发剂，采用沉淀聚合法成功合成了MIPs，傅立叶变换红外光谱(FTIR)分析结果显示MIPs制备良好。因此，此方法可用于对甾烷类化合物具有特异性选择能力的MIPs功能单体的筛选及其制备，并为深层—超深层烃源岩油源对比研究提供技术支持。

To prepare molecularly imprinted polymers (MIPs) with specific selectivity for steranes, the prepolyme-rization system was screened by UV spectroscopy to determine the type, proportion and mode of action of functional monomers. In this study, the interaction intensities between three template molecules (cholesterol, β-sitosterol, deoxycholic acid) and four functional monomers (acrylic acid (AA), methacrylic acid (MAA), methyl methacrylate (MMA), and acrylamide (AM)) are compared respectively. The results show that the functional monomer AA can interact strongly with the three template molecules and form a stable prepolymerization system, so AA is selected as the functional monomer of MIPs. In addition, the UV spectral absorbance change and differential UV spectral analysis of different proportions of AA show that the optimal concentration ratios of the three template molecules to functional monomer AA are all 1:4, and the stable complex configurations formed are cholesterol-1AA, β-sitosterol-1AA and deoxycholic acid-3AA, respectively. The MIPs are successfully synthesized by precipitation polymerization using EDGMA as dispersant and AIBN as initiator, and the results of FTIR show that MIPs are well prepared. Therefore, this method can be used for the screening and preparation of MIPs functional monomers with specific selectivity for steranes, and provide technical support for the study of oil-source correlation between deep and ultra-deep source rocks.

国家自然科学基金 42072180;国家自然科学基金 41772147;国家自然科学基金 41272147;

https://doi.org/10.11781/sysydz202303537