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塔里木盆地新和地区低幅度构造油气微生物特征及有利目标区预测
石油实验地质
2020年 42卷 第6期
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Title
Microbial characteristics of low-amplitude structures and prediction of favorable target areas in Xinhe area, Tarim Basin
Authors
YAN Liang
JIA Baoqian
JI Miao
CHEN Xiaotong
GAO Ping
单位
中国石化 石油物探技术研究院, 南京 211103
中国科学院 微生物研究所, 北京 100101
中国科学院大学, 北京 100049
Organization
SINOPEC Geophysical Research Institute, Nanjing, Jiangsu 211103, China
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
University of Chinese Academy of Sciences, Beijing 100049, China
摘要
对塔里木盆地新和地区油气微生物异常特征进行了研究，为该地区油气预测和油气微生物勘探技术的推广和应用提供依据。主要采用平板培养计数法、16SrRNA基因克隆文库法与高通量测序法对土壤样品中的油气微生物进行定量检测，利用这些方法研究了新和地区油气微生物种群构成及数量异常情况，构建出研究区油气区的油气微生物异常模式。以此为依据，对新和及三道桥地区低幅度圈闭线索区域的油气微生物进行检测，根据油气微生物异常特征预测油气有利区。最终识别出5个微生物异常区，并结合构造圈闭认识结果及油气微生物数量异常，优选出5个潜在的有利勘探目标区。
Abstract
The Microbial Prospecting for Oil and Gas (MPOG) is an exploration method based on the seepage of light gaseous hydrocarbons from oil/gas reservoirs to the surface and their utilization by hydrocarbon oxidizing bacteria. By studying the abnormal characteristics of oil and gas microorganisms in Xinhe area of the Tarim Basin, this project provides a solid scientific basis for oil and gas prediction and the validation and application of oil and gas microbial exploration technology in this area. Soil samples were collected to investigate prospects for hydrocarbon exploration. The amount of cultivable soil microorganisms in different soil samples were analyzed using the agar plating method. High-throughput sequencing has been used directly to study the composition of microbial population in soil samples. Based on the data from Xinhe area, an abnormal pattern of oil and gas microorganisms has been established. By using this pattern, five abnormal microbial areas were identified in the Xinhe and Sandaoqiao area. Combined with the results of structural traps and the abnormal number of hydrocarbon microorganisms, five potential favorable exploration target areas were identified.
关键词：
甲烷氧化菌;
丁烷氧化菌;
平板培养法;
高通量测序;
油气微生物勘探;
新和地区;
塔里木盆地;
Keywords:
methane-oxidizing bacteria;
butane-oxidizing bacteria;
plating method;
high-throughput sequencing;
Microbial Prospecting of Oil and Gas (MPOG);
Xinhe area;
Tarim Basin;
基金项目
国家科技重大专项 2017ZX05005001-009;中石化科技部项目 P18095-4
DOI
https://doi.org/10.11781/sysydz2020061001
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