Title

Microbial diversity above a shale gas field using high-throughput sequencing

作者

顾磊许科伟汤玉平杨帆王国建杜伟高波腾格尔

Authors

GU Lei XU Kewei TANG Yuping YANG Fan WANG Guojian DU Wei GAO Bo BORJIGIN Tenger

单位

中国石化石油勘探开发研究院无锡石油地质研究所, 无锡 214126 中国石化石油勘探开发研究院非常规资源研究所, 北京 100083 中国地质调查局油气资源调查中心, 北京 100083

Organization

Wuxi Research Institute of Petroleum Geology, SINOPEC, Wuxi, Jiangsu 214126, China Research Institute of Unconventional Resources, SINOPEC Petroleum Exploration & Production Research Institute, Beijing 100083, China Oil and Gas Survey, China Geological Survey, Beijing 100083, China

摘要

微生物油气勘探技术近年来已成为油气地表勘探技术中新兴的勘探检测技术。为进一步了解油气田上方的微生物信息，对页岩气区上方微生物多样性进行了研究。采用高通量测序技术研究了川东南丁山地区油气微生物物种丰度、群落多样性等特征。鉴定出了3种主要油气微生物：甲基嗜氧反硝化菌（Methylophilales）、甲基孢囊菌（Methylocystis）、甲基球菌（Methylococcus），值得进一步做生化研究，可以作为潜在油气微生物指示菌。3种微生物发育丰度与丁山地区逸散区域分布具有较好的匹配关系。高通量解析技术能够在丁山地区油气微生物研究中有很好的应用，得出了基岩样品中微生物分布的特征，并与焦石坝地区油气微生物进行了初步的差异性分析，鉴定出的潜在油气指示菌值得进一步研究。

Abstract

In recent years, microbial analysis has become a new detection technology in oil and gas surface exploration. The microbial diversity above a shale gas field was studied in order to further understand microbial information. High-throughput sequencing technology was used to study the abundance and diversity of hydrocarbon species in a shale gas field in the Dingshan area of the southeastern Sichuan Basin. Three main microorganisms were identified, including Methylophilales, Methylocystis, and Methylococcus, which are worthy of further biochemical research and can be used as potential oil and gas indicating bacteria. The developmental abundance of three kinds of microorganisms has a good matching relationship with the distribution of the fugitive area in Dingshan. The high-throughput analytical technology can be applied well in the study of oil and gas microorganisms in Dingshan area, and the distribution characteristics of microorganisms in bedrock samples were obtained. Furthermore, a preliminary analysis of the differences between oil and gas microbes in Dingshan and Jiaoshiba areas was made, indicating that the identified potential oil and gas indicating bacteria deserve further study.

关键词：

油气微生物勘探; 高通量测序; 群落结构; 微生物多样性;

Keywords:

microbial exploration for oil and gas; high-throughput sequencing; community structure; microbial diversity;

基金项目

国家科技重大专项 Z017ZX05036-002;国家自然科学基金 U1663202;国家自然科学基金 41690133;国家自然科学基金 41872126

DOI

https://doi.org/10.11781/sysydz202003443

基于高通量测序技术研究页岩气区上方微生物多样性