新疆陆9 井区砂岩油藏廉价营养体系筛选与评价

Screening and Evaluation of Cheap Nutrient System for Sandstone Reservoir in Lu 9 Well Block of Xinjiang

王红波 连泽特 曹强 范赛华 刘晓丽 马挺

WANG Hongbo LIAN Zete CAO Qiang FAN Saihua LIU Xiaoli MATing

针对陆梁油田陆9 井区砂岩油藏主要采油功能菌的营养需求，以工业发酵副产品和农业废弃物为激活剂的 主要原料，辅助添加关键性营养生长因子组成营养体系。通过单因素实验筛选出最佳碳源、氮源、磷源，初步确 定各组分浓度；以此为基础再通过Plackett-Burman 实验和去因子实验分析各组分的显著性影响因素，确定各组 分的浓度；然后通过响应面实验，根据响应因素对各组分进一步优化，获得最终配方；最后模拟陆9 井区油藏条件 开展物理模拟驱油实验。结果表明，筛选出的营养体系高效、价廉，配方组成为0.15%农副产品加工废料、0.6% 无机盐（含氮量为16.47%）、0.25%无机盐（含氮量为26.16%）。该体系乳化原油效果较好，可激活主要采油功能 基因烃氧化基因拷贝数达到107 copies/mL。物理模拟驱油实验中注入0.4 PV营养体系，同时按气液体积比8∶1 注入空气，可提高采收率11.65 百分点。与现场在用配方相比，优化配方可降低成本20.25%。激活体系的筛选和 评价方法流程为营养体系的筛选评价提供借鉴。

According to the nutrient requirement of the main oil recovery functional bacteria in the sandstone reservoir of Lu 9 well block，by-products of fermentation and agricultural wastes were selected as the main component of the nutrient medium，which also contained some auxiliary microbial growth factors. The optimum carbon source，nitrogen source and phosphorus source were selected through single factor experiment. The concentration of each component was preliminarily determined. On this basis，the significant influence factors of each component were analyzed by Plackett-Burman experiment and factor removal experiment，then the concentration of each component was determined. Finally，response surface test was used to further improve the concentration of each component. Moreover，oil displacement experiment was performed under simulated reservoir condition. The results showed that the nutrient system was efficient and cheap. The nutrient medium contained 0.15% by-products of fermentation and agriculture， 0.6% mineral salt with nitrogen content of 16.47%，and 0.25% mineral salt with nitrogen content of 26.16%. The oil emulsifying effect of the system was good. The copy number of hydrocarbon oxidation gene，which could activate the main oil recovery function gene，reached 107 copies/mL. In the physical simulation oil displacement experiment，the recovery factor could increase 11.65 percentage points after injecting 0.4 PV nutrient system and air at a gas-liquid volume ratio of 8∶1. Compared with the formula used in the field，the optimum formula could reduce the cost by 20.25%. The screening and evaluation process of activation system could provide reference for the screening and evaluation of nutritional system.

10.19346/j.cnki.1000-4092.2022.03.018