硫化物生物抑制剂体系筛选及应用*

Screening and Application of Sulfide Biological Inhibition System

邓舒元 王 博 孙珊珊 白拉贝 佘跃惠 张 凡

DENG Shuyuan WANG Bo SUN Shanshan BAI Labei SHE Yuehui ZHANG Fan

为了抑制油田污水中硫化物的产生，以丘陵站污水为研究对象，实验室中通过监测生物抑制过程培养物中 H2S 含量、SO42- 消耗量、硫酸盐还原菌（SRB）数量优选最佳硫化物生物抑制剂体系。将该体系用于吐哈油田丘 陵站硫化氢抑制矿场试验，并监测加入生物抑制剂体系前后微生物群落变化。研究结果表明：适用于吐哈油田 丘陵站硫化氢生物抑制最佳体系为200 mg/L硝酸钠和60 mg/L JHB，矿场试验过程中硫化物含量均低于1 mg/L， SRB数量低于102个/mL。矿场试验前油田中微生物主要是SRB-嗜热脱硫微球菌（Desulfomicrobium thermophilum）， 矿场试验后假单胞菌（Pseudomonas balearica）和施氏假单胞菌（Pseudomonas stutzeri）在油田污水中占主导地位， 表明生物抑制硫化物的机理是通过生物竞争法争夺SRB的电子受体来抑制SRB的活性，减少油田污水中SRB 数量。图1 表4参22

In order to inhibit the production of sulfides in oilfield wastewater，the wastewater from hilly station was taken as the research object，the optimal sulfides bioinhibitor was optimized by monitoring the H2S content，SO42-consumption and SRB quantity in bioinhibition process culture in laboratory. The system was used in the oilfield test of hydrogen sulfide inhibition in the hilly station of Tuha oilfield，and the microbial community changes before and after adding the bioinhibitor were monitored. The results showed that the optimal hydrogen sulfide biological inhibition system was 200 mg/L sodium nitrate and 60 mg/L JHB，and the sulfide content was less than 1 mg/L and the SRB count was less than 102 cfu/mL in the field test. The main microorganism in the oil field before the field test was desulfomicrobium thermophilum，while pseudomonas balearica and pseudomonas stutzeri dominated in the oilfield wastewater in the oilfield test，indicating that the mechanism of biological inhibition of sulfide is to inhibit the activity of SRB by competing for the electron receptor by biological competition and decreasing SRB in oilfield wastewater.

10.19346/j.cnki.1000-4092.2021.03.030