细菌瓶法用于反硝化菌菌量的测定

Determination of Denitrifying Bacteria Using Bacterial Bottle Method

易绍金 邢燕青 余 灿

YI Shaojin，XING Yanqing，YU Can

在微生物采油（MEOR）研究、菌液产品质量检测、现场试验监控及环保领域，采用费时费事的最大概率数法（MPN法）测定反硝化菌（DNB）菌量。为了更简便准确的测定DNB的菌量，推荐使用含碳源、氮源、磷源及化学脱氧剂等培养液的DNB专用测试瓶。结果表明，将欲测水样注入测试瓶逐级稀释，并在30℃培养5 d后，培养液变浑浊并出现气泡表示有DNB菌生长。DNB的计数按常规细菌瓶法进行。用该细菌瓶法测定了某油田采出液、生物抑菌剂及微生物清防蜡菌剂中DNB菌数，所得结果与MPN法基本相同。与传统的MPN法相比，细菌瓶法具有生长指示明显、操作简单和工作效率高等优点。

In the field of microbial enhanced oil recovery（MEOR）research，bacterial liquid product quality testing，field test monitoring and environmental protection，the amount of denitrifying bacteria（DNB）was determined by the time-consuming most probable number method（MPN method）. In order to measure the amount of DNB bacteria more easily and accurately，it was recommended to use a DNB-specific test bottle containing carbon source，nitrogen source，phosphorus source and chemical deoxidizer. The results showed that the water sample was infused into the test bottle and was diluted step by step，the culture solution became cloudy and bubbles appeared after culturing for 5 days at 30℃，indicating the growth of DNB bacteria. The DNB count was performed according to the conventional bacterial bottle method. The amounts of DNB bacteria in an oilfield production fluid，biological bacteriostatic agent and microbial anti-wax agent were determined by the bacterial bottle method，and the results were basically the same as those of the MPN method. Compared with the traditional MPN method，the bacterial bottle method had the advantages of obvious growth indication，simple operation and high work efficiency.

10.19346/j.cnki.1000-4092.2019.03.014