克拉玛依油田七中区微生物驱微生物功能基因与采油效果的关系

The Relationship between Microbial Functional Genes and Oil Production Performance during Microbial Flooding Process in District Min-7 of Karamay Oilfield

王红波 李明 连泽特 代学成 高配科 马挺

WANG Hongbo LI Ming LIAN Zete DAI Xuecheng GAO Peike MATing

为揭示油藏内源微生物功能基因丰度变化与采油效果的对应关系，利用实时荧光定量PCR检测技术，对克拉玛依油田七中区3 类典型采油井微生物驱产出液中的采油功能菌烃氧化菌（HOB）、硝酸盐还原菌（NRB）和硫酸盐还原菌（SRB）的功能基因alkB、napA和dsrB进行了动态跟踪监测，并与同期采油井的生产开发状况（日产液、产油和含水）结合，分析三类采油功能微生物类群与油井增油降水间的相关性。结果表明：微生物驱后，各试验井有益的HOB增幅较大，其丰度和含量与增油量存在一定的对应关系；NRB丰度显著提高，同时有害菌SRB得到有效抑制。实时荧光定量PCR检测技术可替代传统测试瓶分析技术，实现采油功能菌的快速跟踪监测。图4 表2 参13

In order to reveal the relationship between microbial functional gene numbers and oil production performance during an indigenous microbial enhanced oil recovery（IMEOR）field trial that was performed in district Min-7 of Karamay oilfield，the numbers of the functional genes，including alkB of hydrocarbon oxidizing bacteria（HOB），napA of nitrate reducing bacteria（NRB），and dsrB of sulfate reducing bacteria，were quantified by real-time fluorescent quantitative PCR method. The relationships between the changes of microbial functional gene abundances and oil production performance（liquid production，oil production， and water content）were comparatively analyzed during the IMEOR process. The results showed that the abundance of HOB and NRB increased，while that of SRB decreased，suggesting that the changes of microbial functional gene abundances had a positive correlation with the oil increment. Molecular quantification of microbial functional genes by real-time fluorescent quantitative PCR method could replace the test bottle that based on most probable number（MPN）method，and monitor microbial dynamics during IMEOR process in shorter period of time.

10.19346/j.cnki.1000-4092.2016.04.031