微生物采油过程中鼠李糖脂的快速定量分析

Rapid Quantitative Analysis of Rhamnolipid in Microbial Enhanced Oil Recovery Process

张聪 白罚利 包木太 李一鸣 陆金仁 宋永亭 曹嫣镔 李希明

ZHANG Cong BAI Fali BAO Mutai LI Yiming LU Jinren SONG Yongting CAO Yanbin LI Ximing

在微生物采油过程中对驱油性能进行追踪、监测和评价非常重要，生物表面活性剂的快速定量分析成为一个重要的技术支持。为快速定量测定低浓度的鼠李糖脂，通过对苯酚硫酸法、排油圈法、高效液相色谱质谱联用法进行优化，分别得到了测量鼠李糖脂的标准曲线。结果表明：苯酚硫酸法最佳的酸化时间为1 h、酸化温度为80℃，该法可快速测定质量浓度为100～1000 mg/L的鼠李糖脂，吸光度（A）与鼠李糖脂质量浓度（c）的关系为A=7.465×10-4 c+0.047，线性关系良好（R2=0.9939）；排油圈法可以快速粗略测定浓度为20～500 mg/L的鼠李糖脂，鼠李糖脂质量浓度—排油圈直径标准曲线的R2小于0.95，误差较大；高效液相色谱质谱联用法可以准确测定1～150 mg/L的鼠李糖脂，吸收峰面积S与c的关系为S=3085.4c-2201.4，R2=0.9993，线性关系良好。苯酚硫酸法、排油圈法可用于鼠李糖脂现场快速定量分析，高效液相色谱质谱联用法可用于室内精确定量分析。图7表1参20

The tracking，monitoring and evaluation of flooding performance in microbial enhanced oil recovery process was particularly important，so rapid quantitative analysis of biological surfactant became an important technical support. In order to rapid quantitative determination of rhamnolipid with low concentration，standard curve of rhamnolipid was obtained by optimized phenol-sulfuric acid method，oil spreading method and high performance liquid chromatography-electrospray mass spectrometry（HPLC-MS）. The results showed that the optimum acidification condition of phenol-sulfuric acid method was obtained as follows: 1 h acidification time and 80℃ acidification temperature. The phenol-sulfuric acid method could quickly quantitative measure 100—1000 mg/L rhamnolipid. The relationship of absorbance（A）and rhamnolipid concentration（c）was A=7.465×10-4 c+0.047，with 0.9939 R2，showing good linear relationship. Rhamnolipid with concentration of 20—500 mg/L could be quickly approximate quantification by oil spreading method. The R2 of rhamnolipid concentration and oil zone diameter standard curve was less than 0.95，showing large error. Rhamnolipid with concentration of 1—150 mg/L could be determined accurately by HPLC-MS. The relationship between the absorption peak area S and c was S=3085.4c-2201.4，with 0.9993 R2，showing good linear relationship. Phenol-sulfuric acid method，oil spreading method could be used to fast quantitative analysis of rhamnolipid on site，while HPLC-MS could be used to accurate quantitative analysis indoors.

10.19346/j.cnki.1000-4092.2016.04.034