本源微生物在多孔介质中的代谢特征与驱油效果研究

Study on Metabolite Characteristcs of Indigenous Microorganisms and Enhancement of Oil Recovery in Porous Medium

王俊

WANG Jun

针对大庆二厂水驱油藏开展微生物采油室内试验和评价，优选了乳化效果好的本源微生物菌株Rr（Rhodococcus ruber），研究了Rr 的生理、生化特性，分析了Rr 作用前后原油饱和烃组分，模拟油藏条件监测本源微生物群落的变化与代谢物（有机酸）特征，并进行了微生物驱油实验。研究结果表明，Rr 对石油烃有很好的乳化效果，油水界面乳化活性>60%；作用后原油中的C11～C17 饱和烃浓度降低；模拟驱替产出液中未检测到Rr，但有乙酸、丙酸、丙二酸、乳酸、柠檬酸、琥珀酸等小分子有机酸，其中乙酸浓度可达166.191 mg/L。驱油结果表明，注气补氧不能起到有效提高采收率的作用，注入高浓度的Rr 很可能会打破原有的生态平衡，注入无机盐培养基就能很好地激活油藏微生物，提高采油率8.51%。因此，大庆二厂在进行微生物强化水驱时宜选择本源微生物驱。图5 表2 参10

based on the reservoir condition of the second plant of DaQing oilfield after waterflooding，microbial enhanced oil recovery experiment was developed，and the Indigenous microbial strains Rhodococcus rubber（Rr）with better emulsification was selected，and its physiology and biochemistry characteristic were studied，the saturated hydrocarbon compositions of the crude oil before and after biochemical reaction were analyzed，and the changes of the indigenous microorganism and metabolites were measured with the physical model；the microbial flooding experiments were conducted. The results showed that Rr had better emulsification to oil and paraffin，the emulsification activity of oil-water interface was higher than 60%；the saturated hydrocarbon concentration of nC11—nC17 decreased after microbial reaction；no Rr was checked from the liquid of physical models during incubation；acetic acid，propionic acid，propanedioic acid，citric acid，succinic acid，etc，were evaluated with liquid chromatography， and the concentration of acetic acid was up to 166.19 mg/L. The results of microbial displacement experiment showed the air injection could not enhance oil recovery effectively，the injection of excessive Rr could disturb the Microorganisms' ecological equilibrium，and the simple injection of mineral medium could activate the Microorganisms well and obtain higher enhancement of oil recovery（8.51%）. Accordingly，indigenous microbial flooding after water flooding was suggested in the second plant of Daqing oilfield.

10.19346/j.cnki.1000-4092.2016.03.026