响应面法优化硫酸盐还原菌培养条件

Optimization of Culture Conditions for Sulfate-Reducing Bacteria by Response Surface Methodology

樊月 秦芳玲 王倩 艾晨 张云哲 屈撑囤

FAN Yue，QIN Fangling，WANG Qian，AI Chen ，ZHANG Yunzhe，QU Chengtun

采用Box-Behnken 中心组合设计及响应面法（Response surface methodology）对影响硫酸盐还原菌（SRB）生长的半胱氨酸含量、初始pH 值和Na2SO4含量3个主要因子的最佳水平及其交互作用进行了研究与探讨，并经Design-Expert.8.05B软件分析数据。结果表明：影响SRB菌株反硫化率的主次因素依次为：半胱氨酸浓度、初始pH值、硫酸钠浓度；三因素中两两因素的交互作用均不显著；最佳培养条件为半胱氨酸质量分数0.01%、初始pH值8.0、硫酸钠质量浓度2.0 g/L。在此条件下，SRB菌株的反硫化率平均值为10.642%，与预测值10.097%接近，说明所建立的模型是切实可行的。图3表3 参22

Box-Behnken and response surface methodology were used to research and probe the best level and mutual effect of cysteine concentration，pH value and Na2SO4 concentration that affected the growth of sulfate-reducing bacteria（SRB）. The data was analyzed by Design-Expert.8.05B software. The results showed that three factors were arranged according to their influence on anti-vulcanization rate of SRB in following order: cysteine concentration，medium pH and sodium sulfate concentration. And the interaction between two factors in fitted regression model had insignificant effect on anti-vulcanization rate of SRB. The optimum culture condition was obtained as follows: 0.01% cysteine mass fraction，8.0 pH value and 2.0 g/L sodium sulfate concentration. Under this condition，the average anti-vulcanization rate of SRB was 10.642%，which was close to the predicted value（10.097%）. Therefore，it was feasible for the established model due to the consistent result between prediction and experiment.