两株细菌的特性及物理模拟驱油试验

Characteristics of Two Strains of Bacteria and Physical Simulation Flooding Experiments

康 宏 赵淑娟 李 玥 李盛贤

KANG Hong，ZHAO Shu-Juan，LI Yue，LI Sheng-Xian

分别采用红外光谱法和高效液相色谱法对菌株HD-322-2和T103代谢产生的成分进行分析，并采用这两种菌株进行了岩心模拟驱油实验。结果表明：菌株HD-322-2和T103属于高产表面活性剂的菌株，产量分别为321mg/L和195 mg/L。在发酵20 h后，菌株T103主要产生小分子有机酸，包括乙酸（1.10 g/L）、丙酸（0.24g/L）和丁酸（1.57 g/L）。物理模拟驱油试验结果表明，菌株HD-322-2和T103有较强的驱油性能，复配使用后在水驱的基础上可提高原油采收率5%～7%。

Metabolic ingredients of the strains of HD-322-2 and T103 were analyzed by Infrared spectrum and high performance liquid chromatography, respectively, and core simulation flooding experiments were conducted using the strains of HD-322-2 and T103. The results showed that the strains of HD-322-2 and T103 could yield a lot of surfactant and the yield of the surfactant were about 321 mg/L and 195 mg/L, respectively. After fermentation for 20 h, the strain T103 mainly produced mini-molecule organic acids, such as acetic acid(1.10 g/L), propionic acid(0.24 g/L)), and butyric acid (1.57 g/L). The experiments of physical simulation flooding showed that the strains of HD-322-2 and T 103 had strong flooding performance and the mixing strains could improve the oil recovery about 5% to 7% based on water flooding.