摘要
室内选择性富集、驯化、分离，从井楼油田污水中筛选出3株微生物菌种，分别为产气菌、烃氧化菌和生物表面活性剂菌，3株菌按接种量1:1:1配伍构建共生代谢体系。与单菌种相比，复合菌代谢产物中的表面活性剂含量由1.7%增至2.2%，产气量由95 mL增至120 mL，pH值由6.1降至5.9。针对井楼油田稠油开展的微生物室内评价实验表明，共生代谢体系对该油藏的适应性良好，稠油乳化降黏效果明显，对原油的降黏率最高可达63.9%，形成的水包油乳状液黏度在100~300 mPa·s之间波动，胶质沥青质含量降低，发酵液表面张力下降9.4%~11.6%。在井楼油田7口稠油油井进行首轮氮气辅助微生物吞吐施工，设计单井菌液处理半径10~15 m，氮气顶替段塞2000~10000 Nm3，菌液2.5~6.0 t，措施后单井产液量增加1.8~9.8倍，产油量由0~0.1 t/d增至最高2.7 t/d，首轮施工生产115 d增油470 t，措施有效率85.7%，效果显著。
Abstract
Three strains chosen by enrichment, taming and separation from Jinglou oilfield water were gas production bacteria, hydrocarbon oxidation bacteria and biological surfactant bacterium. After three strains mixed according to inoculation amount in 1:1:1, the biological surfactant in metabolites increased from 1.7% to 2.2%, gas production increased from 95 mL to 120 mL, and pH value decreased from 6.1 to 5.9. The microbial indoor test results for the crude oil of Jinglou oilfield showed that the symbiont digesting strains had a good adaptability, and emulsification of heavy oil viscosity reduction effect was obvious. The drop sticky rate of crude oil could be as high as 63.9%, the viscosity of emulsion oil-in-water varied from 100 to 300 mPa·s, the content of resin and asphalt decreased, and the surface tension of fermented liquid decreased by 9.4%-11.6%. This cold nitrogen microbial production technology was applied in 7 wells of Jinglou oilfield on site with 10-15 m single well radius, 2000-10000 Nm3 nitrogen replacing slug, 2.5-6.0 t microbial fluid. The results showed that the produced liquid per well increased by 1.8-9.8 times, oil production per day increased from 0-0.1 t to a maximum value 2.7 t, oil increment was of 470 t after 115 days, and the effective rate was of 85.7%.
论文详情