In order to analyze the phenomenon of well interference, an interference test analysis model of multiple fractured horizontal well was built, which considers the interference from adjacent injection wells and production wells on well testing behaviors. The model′s type curve plots include eight flow regimes, and the interference flow regime can be divided into two regimes. In the first interference flow regime, pressure derivative curve will rise, the higher the production rate of the adjacent production wells, the larger the magnitude of the rise on pressure derivative curve. The injection well influence the second interference flow regime obviously, the higher the injection rate of the adjacent injection wells, the larger the magnitude of the decline on pressure derivative curve. When the total injection volume is smaller than the production of production wells, pressure derivative curve will descend and behave as a two-steps horizontal line. Finally, the pressure derivative of the second interference flow will become stable. When the total injection is bigger than the production of production wells, pressure derivative curve will descend all the time. Besides, the period of the interference flow regime is inversely proportional to the distance between fractured horizontal well and adjacent active wells. This work provides an important method for determining the connectivity among wells.