变异系数在油藏微生物激活剂筛选中的应用

Application of variation coefficient in selection of reservoir microorganism activator

李磊¹ 晁群芳¹ 方新湘² 王旭辉¹ 陈爱华²

新疆大学生命科学与技术学院，新疆 乌鲁木齐 830046 中国石油克拉玛依石化公司炼油化工研究院，新疆 克拉玛依 834000

College of Life Science and Technology, Xinjiang University, Urumqi 830046, China Oil Refining and Chemical Research Institute, Karamay Petrochemical Company, PetroChina, Karamay 834000, China

为了筛选克拉玛依油田93#油井内源微生物最佳激活剂配方，采用不同激活剂对油藏微生物进行了选择性激活，对激活后的腐生菌、产甲烷菌、反硝化细菌、硫酸盐还原菌、硫细菌、铁细菌数量进行了检测，同时以变异系数为指标对激活效果进行评价，并结合原油降解率及降黏率进行验证。实验结果表明：当可溶性淀粉为碳源、KNO₃为氮源、K₂HPO₄为磷源，且三者质量浓度分别为10，4，1 g/L时，能够较好地刺激油藏有益内源菌的生长，对硫酸盐还原菌、硫细菌、铁细菌等对采油不利的细菌起到一定的抑制作用，说明变异系数可以作为油藏微生物激活剂配方筛选的评价指标之一，为后续现场试验提供理论基础。

In order to select the best activator formula of indigenous microorganisms for No.93 oil well in Karamay Oilfield, different activator formula were used to activate the reservoir microorganisms selectively. The saprophyte, methane-producing bacteria, denitrifying bacteria, sulfate-reducing bacteria, sulfur bacteria and iron bacteria were detected after activation. The activation effect was evaluated in terms of variation coefficient and was verified, combined with the degradation rate of crude oil and viscosity break rate. Experimental result shows that the growth of beneficial indigenous microorganisms can be activated, and the harmful bacteria for oil recovery, including sulfate-reducing bacteria, sulfur bacteria and iron bacteria, can be inhibited when soluble starch is used as carbon source, KNO₃ as nitrogen source, K₂HPO₄ as phosphorus source, and their concentration is respectively 10, 4, 1 g/L. It is stated that the variation coefficient is regarded as one of evaluation indexes for the selection of the reservoir microorganisms activator formula, which can provide a base for sequent field test.

10.6056/dkyqt201204024