一株提高原油采收率嗜热菌株的性能评价

Performance evaluation of a thermophilic MEOR strain

张瀚奭 段传慧 高光军 程海鹰 王静 张前前

ZHANG Han-shi DUAN Chuan-hui GAO Guang-jun CHENG Hai-ying WANG Jing ZHANG Qian-qian

中国海洋大学海洋化学化工学院, 山东 青岛 266003 胜利油田有限公司采油工艺研究院, 山东 东营 257000

从胜利油田产出液中分离得到一株嗜热菌NA-3,经鉴定为芽胞杆菌属。该菌为革兰氏阳性菌,杆状,兼性厌氧,有运动性,菌体大小为(0.5~0.8)μm×(1.4~2.5)μm。最适生长温度为70℃,最适生长pH为7.0~8.0,耐受矿化度可达10 g/L。能产酸和生物表面活性剂,可使培养液的表面张力从72 mN/m降至34 mN/m,pH值从7.0降至5.2。试验表明,NA-3菌液在物理模拟条件下经多轮次驱替仍能提高采收率,微生物提高采收率累计可达11~15个百分点,在微生物采油方面将有很好应用前景。

A thermophilic bacterium of NA-3 was isolated from produced fluid in Shengli oilfield,and identified that it was a member of Bacillus sp.The NA-3 is Gram-positive,rod-shaped in(0.5-(0.8)μm×(1.4)-2.5)μm,facultative anaerobe and motive.The optimum growth temperature is 70 ℃,pH is 7.0.salinity resist is 10 g/L.NA-3 could reduce nutritive fluid surface tension from 72 mN/m to 34 mN/m,pH from(7.0) to 5.2.Experiment shows that NA-3 can enhance the oil recovery to 11-15 percent,and it has great potential application in microbial enhanced oil recovery.

10.3969/j.issn.1000-7393.2006.02.014