分子印迹聚合物微球制备及其对5胆甾烷吸附性能研究

Preparation of molecularly imprinted polymer microspheres and their adsorption performance for 5-cholestane

原陇苗 马荣 陈建珍 邵媛媛 吴应琴

YUAN Longmiao MA Rong CHEN Jianzhen SHAO Yuanyuan WU Yingqin

1. 中国科学院 西北生态环境资源研究院/甘肃省油气资源勘探与评价重点实验室, 兰州 730000; 2. 中国科学院大学, 北京 100049

1. Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences/Key Laboratory of Petroleum Resources Exploration and Evaluation of Gansu Province, Lanzhou, Gansu 730000, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China

采用沉淀聚合法，以胆固醇、去氧胆酸、β-谷固醇为虚拟模板，丙烯酸(AA)为功能单体，偶氮二异丁腈(AIBN)为引发剂，乙二醇二甲基丙烯酸酯(EGDMA)为交联剂，制备甾烷类分子印迹聚合物(MIPs)和空白分子印迹聚合物(NIP)。采用扫描电子显微镜(SEM)、X射线光谱(XRD)、傅里叶变换红外光谱(FT-IR)和比表面积(BET)表征聚合物形貌和结构，并考察其对甾烷类物质的吸附性能。分析结果表明，甾烷类分子印迹聚合物尺寸均一、分散性好，是表面密布孔穴的球形纳米颗粒。吸附性能研究结果表明，MIPs对5α-胆甾烷的吸附能力明显强于NIP，且三种MIPs相比，去氧胆酸、β-谷固醇分子印迹聚合物对5α-胆甾烷的吸附强于胆固醇分子印迹聚合物。通过吸附动力学研究发现，MIPs对5α-胆甾烷的吸附过程符合准二级动力学模型，主要受化学吸附控制；MIPs和NIP的等温吸附符合Langmuir等温吸附模型和Scatchard模型，表明MIPs对5α-胆甾烷具有特异选择性吸附能力，且吸附过程属于单分子层吸附，最大吸附量为0.735 mg/g。表明胆固醇、去氧胆酸、β-谷固醇三种虚拟分子印迹聚合物均对5α-胆甾烷具有较高的分子识别能力及选择性。

Steroidal molecularly imprinted polymers (MIPs) and non-imprinted polymer (NIP) were prepared by precipitation polymerization method using cholesterol, deoxycholic acid, and β-sitosterol as the virtual templates, acrylic acid (AA) as the functional monomer, azobisisobutyronitrile (AIBN) as the initiator, and ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent. The morphology and structure of the polymers were characterized using scanning electron microscopy (SEM), X-ray spectroscopy (XRD), Fourier-transform infrared spectroscopy (FT-IR), and Brunauer-Emmett-Teller (BET) specific surface area analysis. The adsorption performance for steroidal substances was also investigated. The results showed that the steroidal MIPs were uniformly-sized and well-dispersed spherical nanoparticles with porous surface. Adsorption performance results showed that MIPs had significantly stronger adsorption capacities for 5α-cholestane compared to NIP. Among the three MIPs, deoxycholic acid and β-sitosterol MIPs demonstrated stronger adsorption capacities for 5α-cholestane than that of cholesterol. The adsorption kinetics studies showed that the adsorption process of MIPs for 5α-cholestane was in accordance with the pseudo-second-order kinetic model, which was mainly controlled by chemisorption. The isothermal adsorption of both MIPs and NIP conformed to the Langmuir isothermal adsorption model and the Scatchard model, indicating that MIPs exhibited specific selective adsorption for 5α-cholestane and that the adsorption process was monolayer adsorption, with a maximum adsorption capacity of 0.735 mg/g. The study suggests that MIPs prepared with cholesterol, deoxycholic acid, and β-sitosterol as virtual templates have high molecular recognition and selectivity for 5α-cholestane.

国家自然科学基金(42072180，41772147，41272147)和中国科学院仪器设备功能开发技术创新项目(E0280101)联合资助。

https://doi.org/10.11781/sysydz2024051098